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KMID : 0380020220370010032
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Korean Journal of Biotechnology and Bioengineering
2022 Volume.37 No. 1 p.32 ~ p.40
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Oxygen Transfer Rate (OTR) Prediction Model for CHO Cell Culture in Shake Flasks
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Ko Han-Chul
Yoo Hyun-Wook
Baik Jong-Youn
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Abstract
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Shake flasks are widely used as small-scale mammalian culture vessels during cell line development and bioprocess optimization for the production of biopharmaceuticals. However, the lack of information as well as controllability on dissolved oxygen often leads to oxygen limitation that can adversely affect cell growth and productivity during cell culture, thereby challenging cell line development and scale-up processes. In this work, we report an oxygen transfer rate (OTR) prediction model of shake flasks using Chinese hamster ovary (CHO) cells to avoid oxygen limitation issues in shake flasks. First, we varied shaking speed and culture volume to measure maximum OTR (OTRmax) and created an OTRmax regression function. Then, we estimated theoretical maximum viable cell density with the specific oxygen uptake rate (sOUR) of CHO cells and the OTRmax values. Lastly, we tested if decreasing culture volume or increasing shaker speed could mitigate the adverse effect of oxygen limitation. In conclusion, we believe this OTR prediction model would provide information about the oxygen availability in shake flasks, presumably assisting to achieve better culture performances.
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KEYWORD
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dissolved oxygen (DO) concentration, oxygen transfer rate (OTR), Chinese hamster ovary (CHO) cell, shake flask
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